纳米黏土水凝胶孔径由46微米降至26微米：含2%拉波泥配方7天质量损失由57%降至16%，《科学报告》11月14日刊出
《科学报告》11月14日刊出一项水凝胶研究。研究团队制备了含 0%、1% 与 2% 拉波泥（laponite）纳米黏土的明胶与海藻酸盐水凝胶，评估其作为细胞相容支架材料的形貌、力学、理化与生物学性质。
冷冻干燥后的扫描电镜截面显示，平均孔径随拉波泥含量上升而减小：不含拉波泥的配方为 46±9 微米，含 1% 的为 42±8 微米，含 2% 的为 26±7 微米。
溶胀与降解方面，与不含拉波泥的配方相比，含 1% 与 2% 拉波泥配方的 360 分钟溶胀比分别下降约 29% 与约 47%；7 天质量损失由 57% 分别降至 48% 与 16%。含 1% 拉波泥配方的抗压强度为不含拉波泥配方的两倍以上。
未交联的前驱体配方表现出剪切变稀行为。各配方水凝胶的含水率保持在 90% 以上。研究采用傅里叶变换红外光谱、X 射线衍射、扫描电镜、溶胀与降解分析、压缩与流变测试、MTT 实验与细胞黏附研究进行表征。
生物学测试中，水凝胶支持 L929 成纤维细胞的代谢活性与人骨髓间充质干细胞的初步黏附。论文称，结果显示该材料在受试条件下具有细胞相容性，并有开展进一步细胞研究的潜力。
图中是三条半透明的水凝胶条状样品
Nanoclay Hydrogel Pore Size Falls From 46 to 26 Micrometres as 2 Percent Laponite Cut Seven-Day Mass Loss From 57 to 16 Percent, Scientific Reports, November 14
Scientific Reports published a hydrogel study on November 14. The team fabricated gelatin and alginate hydrogels containing 0, 1 and 2 weight percent laponite nanoclay, and assessed their morphological, mechanical, physicochemical and biological properties as potential cytocompatible scaffold materials.
Scanning electron microscopy of freeze-dried cross-sections showed average pore size shrinking as laponite content rose: 46 plus or minus 9 micrometres with no laponite, 42 plus or minus 8 micrometres at 1 percent, and 26 plus or minus 7 micrometres at 2 percent.
On swelling and degradation, the 360-minute swelling ratio fell by approximately 29 percent for the 1 percent formulation and 47 percent for the 2 percent formulation compared with the laponite-free hydrogel, while seven-day mass loss fell from 57 percent to 48 percent and 16 percent respectively. The 1 percent formulation showed more than twice the compressive strength of the laponite-free hydrogel.
The non-crosslinked precursor formulations exhibited shear-thinning behavior, and the hydrogels maintained hydration above 90 percent. Characterization used FTIR, XRD, SEM, swelling and degradation analyses, compression and rheological testing, MTT assay and cell attachment studies.
In biological testing, the hydrogels supported L929 fibroblast metabolic activity and initial attachment of human bone marrow-derived mesenchymal stem cells. The paper said the results demonstrate cytocompatibility under the tested conditions and potential for further cell-based studies.
来源：《科学报告》
发布：GSM·美国（发布链接）
图片来源：图：三条半透明的水凝胶条状样品。来源：美国国家标准与技术研究院（NIST），公共领域，维基共享资源。 / Photo: Three translucent hydrogel strip samples. Credit: National Institute of Standards and Technology, public domain, via Wikimedia Commons.
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