塞内加尔新生儿血清蛋白质组研究绘出979种蛋白质 53蛋白簇可区分新生儿败血症
《科学报告》11月14日发表一项新生儿败血症蛋白质组研究。论文指出，新生儿败血症是发病与死亡的重要原因，在塞内加尔，发病率为每1000名活产儿78至104例，死亡超过每1000名活产儿20例，病死率约36%。
研究团队用数据非依赖采集蛋白质组学方法，在 Evosep-timsTOF HT 平台上分析塞内加尔新生儿与成人的血清样本，目标是建立新生儿与成人血清蛋白质组的参照，并寻找新生儿败血症的血清蛋白标志物。
研究在新生儿血清中鉴定出979±20种蛋白质，成人为718±40种。新生儿的免疫反应相关蛋白较少、动态范围较窄，胶原等结构蛋白较多，与免疫未成熟和组织发育的特点一致。无监督 WGCNA 分析得到一个由53种蛋白质组成的蛋白簇，可以区分确诊败血症与未确诊败血症及健康对照。
作者把更大的标志物组合归纳为三条生物学通路：病原体感知与急性期炎症、先天免疫激活、白细胞募集与血小板耗竭、内皮损伤与微血管功能障碍及组织重塑，同时伴随保护性脂蛋白和丝氨酸蛋白酶抑制剂的减少。论文由 Roger、Coly、Metatla 等作者发表。
Senegal Newborn Serum Proteome Maps 979 Proteins, 53-Protein Cluster Distinguishes Neonatal Sepsis
Scientific Reports on November 14 published a serum proteomics study of neonatal sepsis. The paper notes that neonatal sepsis is a major cause of morbidity and mortality, particularly in low- and middle-income countries such as Senegal, where incidence is 78 to 104 per 1000 live births, mortality exceeds 20 per 1000, and case fatality rates are around 36 percent.
The team analyzed serum samples from Senegalese neonates and adults using data-independent acquisition (DIA) proteomics on neat serum on an Evosep-timsTOF HT platform, aiming to establish a neonatal-versus-adult reference and to identify serum protein biomarkers of neonatal sepsis.
The study identified 979 ± 20 proteins in newborns versus 718 ± 40 in adults. Newborns showed reduced immune-response related proteins, a narrower dynamic range, and increased structural proteins such as collagens, consistent with immune immaturity and tissue development. Unsupervised WGCNA analysis led to a 53-protein cluster that discriminated confirmed sepsis from non-confirmed sepsis and healthy controls.
The larger marker panel maps to three major biological processes in sepsis: pathogen sensing and acute-phase inflammation; innate immune activation, leukocyte recruitment and platelet exhaustion; and endothelial injury and microvascular dysfunction with tissue remodeling, alongside loss of protective lipoproteins and serpins. The paper is authored by Roger, Coly, Metatla and colleagues.
来源：《科学报告》
发布：GSM·美国（发布链接）
图片来源：图：圣迭戈海军医疗中心的新生儿重症监护病房（NICU）设施（资料图）。来源：美国海军照片，Jake Berenguer 摄，公共领域。 / Photo: The neonatal intensive care unit facility at Naval Medical Center San Diego (file photo). Source: U.S. Navy photo by Mass Communication Specialist 3rd Class Jake Berenguer, Public domain.
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