欧洲海鲈4小时运输应激研究：黏液、鳞片与水体皮质醇均可反映血浆水平
《科学报告》11月14日发表的一项研究考察了欧洲海鲈（Dicentrarchus labrax）在运输应激下的生理和分子反应，并评估微创和无创监测方法。研究由 Simó-Mirabet、Rodríguez-Viera、Molina-Roque 等人完成，论文于7月2日投稿、9月29日被接受。
研究中，海鲈幼鱼被运输4小时，一组在运输后立即取样（TRANSP 组），一组在24小时恢复期后取样（RECOV 组），并设有未受干扰的对照组（CTRL1 和 CTRL2）。研究人员测量了血浆、皮肤黏液、鳞片和水体四种基质中的皮质醇水平。
结果显示出典型的短途运输反应，包括水体酸化、血红蛋白升高和血细胞比容降低；受运输鱼类所有受测基质中的皮质醇均显著升高。黏液、鳞片和水体中的皮质醇与血浆皮质醇呈正相关，证实这些替代基质可作为可靠的微创应激评估工具。
次级应激反应还表现为血浆葡萄糖、甘油三酯和乳酸升高，肝脏葡萄糖和肌肉乳酸耗减；转录层面，头肾中 cxcr4、il10 和 hsp70 上调，脑中 crhbp 和 hsp70 上调。作者据此表示，运输引发的应激反应可以通过微创和无创方法检出。
European Sea Bass Transport Stress Study: Cortisol in Mucus, Scales and Water Tracks Plasma After 4-Hour Transport
A study published in Scientific Reports on November 14 examined the physiological and molecular responses of European sea bass (Dicentrarchus labrax) to transport stress, evaluating less-invasive and non-invasive monitoring methods. The work, by Simó-Mirabet, Rodríguez-Viera, Molina-Roque and colleagues, was received on July 2 and accepted on September 29.
Sea bass juveniles were transported for 4 hours and sampled either immediately after transport (TRANSP group) or after a 24-hour recovery period (RECOV group), alongside undisturbed control groups (CTRL1 and CTRL2). Cortisol was measured across four matrices: plasma, skin mucus, scales and water.
Typical short-transport responses were observed, including water acidification, increased haemoglobin and reduced haematocrit, and cortisol levels increased significantly across all evaluated matrices in the transported fish. Positive correlations between cortisol in mucus, scales and water relative to plasma confirm these alternative matrices as reliable, less-invasive tools for stress evaluation.
Secondary stress responses included higher plasma glucose, triglycerides and lactate, alongside depleted liver glucose and muscle lactate. Transcriptional changes included upregulation of cxcr4, il10 and hsp70 in the head kidney, and crhbp and hsp70 in the brain. The authors concluded that transport triggers clear stress responses that can be detected by less-invasive and non-invasive monitoring.
来源：《科学报告》
发布：GSM·美国（发布链接）
图片来源：图：一条欧洲海鲈（Dicentrarchus labrax）在水中。作者：Julien Renoult，CC BY 4.0，维基共享资源。 / Photo: A European sea bass (Dicentrarchus labrax) underwater. Credit: Julien Renoult, CC BY 4.0, via Wikimedia Commons.
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