盐单胞菌 D23 具群体淬灭能力 12 小时内降解鱼类病原信号分子
11 月 14 日发表于《科学报告》的一项研究评估了盐单胞菌 D23 作为水产养殖益生菌的潜力。研究作者为 Ghosh、Alam 与 Mukhopadhyay。该菌株具有群体淬灭能力，可降解鱼类病原菌用于通讯的信号分子。
实验显示，D23 可耐受 1.2% 的胆盐与 0.75% 的苯酚，在 pH 2.0 至 4.0 的酸性环境中存活；其抗氧化活性高于 69%，自聚集率为 72.0%（±2.42%），与鱼类病原菌的共聚集率高于 73%。D23 在 12 小时内降解了嗜水气单胞菌的天然 N-AHLs 信号分子，共培养抗生物膜效果的 p 值小于等于 0.0001。
基因组分析未检出毒力基因、质粒或转座元件相关基因，仅检出极少数耐药基因；研究还检出一个带「66HXHXDH71」基序的推定内酯酶 lactonase_D23。
Halomonas Strain D23 Shows Quorum-Quenching Activity, Degrading Fish Pathogen Signaling Molecules Within 12 Hours
A study published November 14 in Scientific Reports evaluated Halomonas strain D23 as a candidate probiotic for aquaculture. The authors are Ghosh, Alam, and Mukhopadhyay. The strain has quorum-quenching activity, degrading the signaling molecules that fish pathogens use to communicate.
In experiments, D23 tolerated 1.2 percent bile salt and 0.75 percent phenol and survived acidic conditions of pH 2.0 to 4.0. Its antioxidant activity exceeded 69 percent, auto-aggregation was 72.0 percent (plus or minus 2.42 percent), and co-aggregation with fish pathogens exceeded 73 percent. D23 degraded the natural N-AHLs of Aeromonas hydrophila within 12 hours, and its anti-biofilm effect in co-culture reached a p value of 0.0001 or lower.
Genome analysis detected no virulence genes and no plasmid- or transposon-associated genes, and only very few resistance genes. The study also identified a putative lactonase, lactonase_D23, carrying a 66HXHXDH71 motif.
来源：《科学报告》
发布：GSM·美国（发布链接）
图片来源：图：希腊 Euboea 岛 Amarynthos 近海的鱼类养殖网箱。来源：Jebulon，Wikimedia Commons，CC0 1.0。 / Photo: Fish farming cages off Amarynthos on the Greek island of Euboea. Source: Jebulon, Wikimedia Commons, CC0 1.0.
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