联合干预后血液β淀粉样蛋白42上升约35%：《自然·通讯》论文称舌下硝酸甘油加全身振动与脑源蛋白外排相关，29名健康成年人受试
《自然·通讯》11月3日发表 Tuunanen 等作者的论文《血管机械治疗与人类脑蛋白外排增加相关》。这是一项在健康志愿者中进行的随机交叉试验：共29名健康成年人（其中女性15名，平均年龄44岁，年龄范围27至61岁）参加，接受两种干预，针对 Piezo1 机械敏感通道的全身振动（WBVp，27人）与一氧化氮介导血管舒张联合全身振动（NO+WBVp，26人）；另有12人参加了仅一氧化氮干预的探索性环节。研究团队来自芬兰奥卢大学等机构，试验注册号为 EudraCT 2021-000625-27。
主要结果方面，联合干预后，受试者血液中低分子量（小于68 kDa）脑源蛋白显著上升：血清β淀粉样蛋白40由干预前38.2 pg/ml升至校正后50.5 pg/ml，β淀粉样蛋白42由2.6 pg/ml升至3.5 pg/ml（升幅约35%），胶质纤维酸性蛋白（GFAP）中位数由65.2 pg/ml升至85.1 pg/ml；而分子量更大的神经丝轻链与磷酸化 tau-181 未见变化，作者据此提出分子大小可能是脑脊液向血液转运蛋白的一个因素。干预期间受试者血压下降，收缩压平均下降14.9%、舒张压平均下降14.8%；血压下降幅度与三种蛋白的升幅相关。磁共振方面，联合干预后全脑 T2 黑液（T2DF）信号强度平均下降4.6%，脑外游离脑脊液体积平均增加6.5 ml。
作者口径方面，论文标题与结论均使用「相关」（associated with）表述：该研究测量的是血液生物标志物与影像指标的变化，未测量脑内淀粉样蛋白负荷或认知结果；小鼠鞘内示踪实验为人体观察提供生物学支持。论文为正式发表的同行评审论文，上述数值均为该单次干预试验的实测结果，不构成任何治疗建议。
（来源：《自然·通讯》）
Blood Amyloid-Beta 42 Rose About 35% After a Single Combined Session: A Nature Communications Paper Links Sublingual Nitroglycerin Plus Whole-Body Vibration to Brain Protein Efflux in 29 Healthy Adults
A paper published in Nature Communications on November 3 by Tuunanen and colleagues, titled "Vasomechanic treatment is associated with increased efflux of human brain proteins," reports a randomized crossover trial in healthy volunteers. Twenty-nine healthy adults (15 female; mean age 44 years, range 27-61) took part, undergoing mechanotransductive whole-body vibration targeting the Piezo1 channel (WBVp, n = 27) and the combined protocol of nitric-oxide-mediated vasodilation with WBVp (NO+WBVp, n = 26), with an additional exploratory NO-only session in a subset (n = 12). The team is led from the University of Oulu in Finland, and the trial registration is EudraCT 2021-000625-27.
After the combined intervention, low-molecular-weight (under 68 kDa) brain-derived proteins in blood rose significantly: serum amyloid-beta 40 from 38.2 pg/ml before treatment to a hemodilution-corrected 50.5 pg/ml after, amyloid-beta 42 from 2.6 to 3.5 pg/ml (a rise of about 35%), and glial fibrillary acidic protein (GFAP) from a median of 65.2 to 85.1 pg/ml. The larger neurofilament-light and phosphorylated tau-181 proteins were unaltered, which the authors say suggests molecular weight could be a factor in CNS-to-blood protein movement. Systolic and diastolic blood pressure fell during the intervention by means of 14.9% and 14.8% respectively, and the extent of blood-pressure reduction correlated with the increases in all three proteins. On MRI, whole-brain T2 dark-fluid signal intensity decreased by a mean of 4.6% after the combined treatment, and segmented extracerebral free CSF volume increased by a mean of 6.5 ml.
The paper's title and conclusions use the language of association: the study measured changes in blood biomarkers and imaging signals, and did not measure brain amyloid burden or cognition; complementary intrathecal tracer studies in mice provided biological support. The article is a formally published, peer-reviewed paper; the figures are single-session trial measurements and amount to no treatment recommendation.
(Source: Nature Communications)
来源：《自然·通讯》
发布：GSM·美国（发布链接）
图片来源：图：志愿者接受 PiB PET 扫描以检测脑内淀粉样蛋白（资料图）。CSIRO 摄，CC BY 3.0，经 Wikimedia Commons。 / Photo: A volunteer receiving a PiB PET scan to detect brain amyloid (file photo). Photo by CSIRO, CC BY 3.0, via Wikimedia Commons.
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